Applied Medical Research. 2021;
8(1):(86-176)
Characterization and Immunologic Response of IgY Against Leishmania Mexicana
Marbel Torres-Arias
Abstract
Avian IgYs are polyclonal antibodies resulting from humoral adaptive immune responses.
With perspective for many application, IgYs are considered the most versatile biomolecule,
able to be used as alternative in biomedicine and as diagnostic tool. Because they are
inducible, these antibodies could be produced to potentiate the recognition of relevant
clinical pathogens such as Leishmania mexicana. In this investigation anti Leishmania
mexicana IgYs were obtained by inoculation of inactivated promastigotes in brown
Lohmann hens, at different periods. Twenty-four per cent of the eggs were randomly
selected for processing. IgYs were precipitated by Ammonium Sulfate and purified by affinity
chromatography. Immunoglobulins with 175 kDa, complete structure, and a concentration
of 6.3 mg/mL per yolk were obtained. The most reactive antibodies corresponded to
week 8 (W8). These IgYs were tested for specific recognition of their target parasite, using
structural and proteins viral as GP63 in Western Blot. Also, they identified concentrations of
parasite antigen of 5 and 50 μg/mL with maximal antibody dilution of 1:2000 in ELISA. The
IgY-mediated immunodetection offers this as solution to the necessity of anti L. mexicana
antibodies for diagnostics and immunotherapy research.
